AAV2 ELISA Control

Cat. No: PRAAV2-C

Available, delivery time 1-7 days

$135.00*

Key Features
  • Consists of fully assembled, empty AAV2 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA
Product description
Quantity 1 vial (0.8E+09-1.4E+09 capsids)
Application ELISA
Storage 2-8°C
Intended use Research use only
Concentration

1.7E+09-2.8E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)

Formulation Lyophilized, empty AAV2 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV2 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer.
Stability 4 weeks at 2-8°C after reconstitution in ASSB 1x
Applications
Tested applications Tested dilutions
ELISA As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV2 Titration ELISA) and analysis at least in duplicates is recommended.
Background

The AAV2 ELISA Control consists of fully assembled, empty AAV2 capsids. The concentration is lot specific.

The AAV2 ELISA Control can be used as a positive control with PROGEN's AAV2 Titration ELISA (PRATV & PRAAV2R). If you require different lots of the AAV2 ELISA Control and the Kit Control included in your PROGEN AAV2 Titration ELISA, please enquire to check availability.

Reading of the AAV2 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV2 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.

The AAV2 ELISA Control has been calibrated on the ATCC AAV2 reference standard material (RSM, VR-1616). For further information, please see the publication by Lock et al., 2010.

Publications

Lock M, McGorray S, et al. Characterization of a recombinant Adeno-Associated Virus Type 2 reference standard material; Hum Gene Therapy 21:1273-1285 (2010).


References/Publications (2)
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FAQs
Yes, PROGEN offers serotype-specific AAV ELISA Controls for the standardization of your AAV titer determination. The serotype-specific positive controls are fully characterized empty AAV capsids standardized with PROGEN´s internal gold standards* (AAV1, AAV3, AAV5, AAV6 & AAV9) or the ATCC international gold standard material (AAV2 & AAV8).

*For more information on PROGEN´s internal gold standard, please check out our posters "Developing Reliable AAV Standards for ELISA" and "The New AAV3 Titration ELISA" describing the establishment and characterization of AAV5 and AAV3 internal gold standards.
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  • Ready-made, sterile and endotoxin-tested solution of iodixanol
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  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
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Cat. No: 610298
Quantity: 50 µg
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  • Recombinant antibody
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Cat. No: 61055
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
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AAV2 Titration ELISA
Cat. No: PRATV
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20 antibody is used as capture and detection antibody

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AAV2 Titration ELISA 2.0R
Cat. No: PRAAV2R
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20R antibody is used as capture and detection antibody

$677.00*