AAV2 VP3, recombinant protein



Undiluted = 100 µg/ml, pre-diluted = 10 µg/ml

10 µl of each solution can be separately loaded onto the SDS PAGE and analyzed by Western blot using the B1 antibody (Cat. No. 690058, Cat. No. 61058-488, Cat. No. 61058-647).













Undiluted = 100 µg/ml, pre-diluted = 10 µg/ml

10 µl of each solution can be separately loaded onto the SDS PAGE and analyzed by Western blot using the B1 antibody (Cat. No. 690058, Cat. No. 61058-488, Cat. No. 61058-647).










• Useful in combination with AAV2 VP1 and VP2 for ratio analysis (also available as set, Cat. No. 72001)
• Precise molar concentration
• Suitable for WB, dot blot, CE, SDS PAGE
Product description
Quantity | 10 µg |
---|---|
Application | Capillary electrophoresis (CE), Dot blot, SDS PAGE, WB |
Purification | Ni-NTA chromatography |
Storage | -80°C |
Intended use | Research use only |
Concentration | 100 µg/ml (1.61 µM) |
Formulation | Liquid, 6 M urea in PBS |
Source | Escherichia coli |
Molecular weight | 62.2 kDa (calculated Mw from aa sequence) |
Purity | > 90% (determined by SDS PAGE) |
Product description | N-terminal His-tagged (MGSSHHHHHHSSGLVPRGSH) recombinant AAV2 capsid protein VP3 |
Applications
Tested applications | Tested dilutions |
---|---|
Capillary electrophoresis (CE) | Assay dependent |
Dot Blot | 100 ng, depending on primary antibody and detection method |
SDS PAGE | 1 µg |
Western Blot (WB) | 5-20 ng, depending on primary antibody and detection method |
Background
All three recombinant AAV2 capsid proteins are available as set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).
Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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