Keratin K14, human recombinant, 100 µg

100 µg

250 µg

Cat. No: 62218

Prices excl. tax

Available, delivery time: 1-7 days

$364.00*

Datasheet

Key Features

Recombinant human keratin K14 (formerly also designated cytokeratin 14)
Protein standard

Product description

Quantity	100 µg
Storage	Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use	Research use only
Source	Human recombinant, produced in E. coli
Molecular weight	50 kDa
Isoeletric point	pI 5.3
Purity	> 95% (determined by SDS gelelectrophoresis)
Reconstitution	Reconstitute with 70 µl distilled water (final volume 100 µl). Final solution: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride; Protein concentration: 1 mg/ml
Application	Protein standard in 1D and 2D SDS gelelectrophoresis, immunoassays and immunization
Synonym	Cytokeratin 14

Background

Human recombinant Keratin K14 for use in immunoblotting and ELISA.

Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255

Downloads

Datasheet

MSDS

Progen Support

For scientific and customer support, you can reach us by phone or email.

Learn more

Related Products

anti-Keratin K20 mouse monoclonal, IT-Ks20.8, liquid, purified, sample

Cat. No: 690026S

Quantity: 200 µl (50 µg/ml)

Purified, liquid, small
Mouse monoclonal
Suitable for IHC and WB
Reacts with human and mouse
Isotpye: IgG2a

$96.00*

anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), liquid, purified, sample

Cat. No: 690010S

Quantity: 200 µl (50 µg/ml)

Purified, liquid, small
Mouse monoclonal
Suitable for ELISA, ICC/IF, IHC and WB
Reacts with human
Isotype: IgG2a

$96.00*

anti-Keratin K7 mouse monoclonal, Ks7.18, liquid, purified, sample

Cat. No: 690025S

Quantity: 200 µl (50 µg/ml)

Purified, liquid, small
Mouse monoclonal
Suitable for ICC/IF, IHC and WB
Reacts with bovine, human, pig and sheep
Isotype: IgG1

$96.00*

anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant

Cat. No: 65191

$179.00*

anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant

Cat. No: 65177

Quantity: 1 ml

Hybridoma cell culture supernatant
Mouse monoclonal
Suitable for IHC and WB
Reacts with human
Isotype: IgG1

$179.00*

anti-Keratin K19 mouse monoclonal, Ks19.10, supernatant

Cat. No: 65135

$191.00*

anti-Keratin K18 mouse monoclonal, Ks18.5, supernatant

Cat. No: 65137

$191.00*

anti-Keratin K8 mouse monoclonal, Ks8-17.2, supernatant

Cat. No: 65130

$191.00*

anti-Keratin K7 + K17 mouse monoclonal, C46, supernatant

Cat. No: 18313

$215.00*

anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant

Cat. No: 18315

$215.00*

anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified

Cat. No: 610145

$216.00*

anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified

Cat. No: 65031

Quantity: 5 ml

Purified, prediluted
Mouse monoclonal
Suitable for IHC and WB
Reacts with bovine, human, mouse and rat
Isotype: IgG1

Variants from $96.00*

$220.00*

anti-Keratin Pan rabbit polyclonal, purified

Cat. No: 10550

$232.00*

anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant

Cat. No: 651104

$245.00*

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified

Cat. No: 690029

Quantity: 1 ml (50 µg/ml)

Purified, liquid
Mouse monoclonal
Suitable for ELISA, ICC/IF, IHC and WB
Reacts with bovine, human, rabbit, rat
Isotype: IgG2b

Variants from $96.00*

$251.00*

anti-Keratin K8 mouse monoclonal, Ks8.7, liquid, purified

Cat. No: 690038

Quantity: 1 ml (50 µg/ml)

Purified, liquid
Mouse monoclonal
Suitable for ICC/IF, IHC and WB
Reacts with hamster and human
Isotype: IgG1

Variants from $96.00*

$251.00*

anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified

Cat. No: 61007

Quantity: 50 µg

Purified, lyophilized
Mouse monoclonal
Suitable for ICC/IF, IHC and WB
Reacts with bovine, human, rat
Isotype: IgG1

Variants from $161.00*

$251.00*

anti-Keratin K18 mouse monoclonal, Ks18.27, lyophilized, purified

Cat. No: 61034

$251.00*

anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified

Cat. No: 61090

Quantity: 50 µg

Purified, lyophilized
Mouse monoclonal
Suitable for IHC and WB
Reacts with human, mouse and rat
Isotype: IgG1

Variants from $96.00*

$251.00*

anti-Keratin K17 mouse monoclonal, Ks17.E3, liquid, purified

Cat. No: 690036

Quantity: 1 ml (50 µg/ml)

Purified, liquid
Mouse monoclonal
Suitable for IHC and WB
Reacts with human, rat
Isotype: IgG2b

Variants from $96.00*

$251.00*

anti-Keratin K18 Multiepitope Cocktail mouse monoclonal, Ks18.04 + 18.27 + 18.5 + 9B1 + 18.174, supernatant

Cat. No: 651134

$258.00*

anti-Keratin Type I mouse monoclonal, AE1, purified

Cat. No: 61804

$284.00*

anti-Keratin Type II mouse monoclonal, AE3, purified

Cat. No: 61806

$284.00*

anti-Keratin K13 mouse monoclonal, 2D7, supernatant

Cat. No: 10524

$285.00*