Epitope mapped

anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified

Liquid

Liquid

Lyophilized

Prediluted

Biotin

FITC

Cat. No: 65031

Available, delivery time 1-7 days

$220.00*

Key Features
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with amphibia, bovine, human, hydra, mouse and pig
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Amphibia, Bovine, Human, Hydra, Mouse, Pig
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks 5+8.22/C22
Immunogen Human keratin K8, purified from SDS PAGE gel
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID Q5XQN5 (Bovine), Q7RTS7 (Human), Q922U2 (Mouse), A0A287BA49 (Pig), P05786 (Bovine), P05786 (Bovine), P05787 (Human), P11679 (Mouse)
Synonym Keratin, type II cytoskeletal 74, Cytokeratin-74, CK-74, Keratin-5c, K5C, Keratin-74, K74, Type II inner root sheath-specific keratin-K6irs4, Type-II keratin Kb37, KRT74, K6IRS4, KB37, KRT5C, KRT6IRS4, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia.

Polypeptide reacting: Mr 52,500, Mr 58,000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al., 2004).

Reactivity on cultured cell lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).

References/Publications (1)
Publication
Species
Application
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Category Protocol
Size 924.86 KB
Filetype pdf
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anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, RCK102, supernatant
Cat. No: 10521
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with dog, hamster, human, mouse, pig, rabbit and rat
  • Isotype: IgG1

$285.00*