Conformational epitope
Highly published
Neutralizing

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

Lyophilized

Lyophilized

Supernatant

Biotin

Cat. No: 61055

Available, delivery time 1-7 days

$461.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for dot blot, ELISA, ICC and neutralization assay
  • Reacts with AAV2, AAV2 7m8 and AAV3 intact particles
  • Isotype: IgG3
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG3
Conjugate Unconjugated
Application Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay
Purification Affinity chromatography
Reactivity AAV2, AAV2 7m8, AAV3
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution 2-8°C
Intended use Research use only
Clone A20
Immunogen AAV2 capsids
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym Adeno-associated virus 2; AAV-2
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Affinity Chromatography Assay dependent
Dot Blot 1:500 (0.1 µg/ml; non-denaturing conditions)
ELISA Assay dependent
Immunoprecipitation (IP) 1:5
Neutralization Assay Assay dependent
Background

For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20 specifically reacts with AAV2 and AAV3, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
Epitope mapping experiments (Wobus et al. 2000) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV2 capsids. The antibody is also useful for neutralizing experiments.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281-93 (2000).
Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761-1766 (2000).

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (11)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

A20 is a mouse IgG3 monoclonal antibody, while A20R is recombinant mouse IgG1 antibody. Both antibodies recongize the same epitope, only the antibody backbone is different.
There is no difference in performance.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
AAV2 ELISA Control
Cat. No: PRAAV2-C
  • Consists of fully assembled, empty AAV2 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
OptiPrep™ (Iodixanol)
Cat. No: 1114542
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

$195.00*
anti-AAV2 (intact particle) mouse recombinant, A20R, lyophilized, purified
Cat. No: 610298
Quantity: 50 µg
  • Purified, lyophilized
  • Recombinant antibody
  • Suitable for dot blot, ELISA, neutralization assay
  • Reacts with AAV2, AAV3, AAV2 7m8 and Anc80 intact particles
  • Isotype: mouse IgG1

Variants from $131.00*
$461.00*
AAV2 Titration ELISA
Cat. No: PRATV
  • ELISA for quantitation of AAV2 capsids
  • Detection of full and empty AAV2 capsids
  • A20 antibody is used as capture and detection antibody

$677.00*