anti-acidic Hair Keratin K32 guinea pig polyclonal, serum

Cat. No: GP-HHA2

Available, delivery time 1-7 days

$325.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human
No reactivity Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide of human hair (trichocytic) keratin K32 (formerly also designated keratin hHa2; C-VTRTVCVPRTVGM), coupled to KLH
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q14532 (Human)
Synonym Keratin, type I cuticular Ha2, Hair keratin, type I Ha2, Keratin-32, K32, KRT32, HHA2, HKA2, KRTHA2
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:200 (for enhancement of cortex staining preincubate fixed sections with 0.1% Triton X-100 (in PBS) for 1-5 min prior to first antibody incubation step)
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and microwave treatment recommended; for enhancement of cortex staining preincubate fixed sections with 0.1% Triton X-100 (in PBS) for 1-5 min prior to first antibody incubation step)
Western Blot (WB) 1:2,000
Background

The antiserum stains specifically human hair keratin K32 expressed in the hair cuticle (Mr 45,000 polypeptide) and tumors derived from.

References/Publications (3)
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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