Frequently used
Diagnostic marker

anti-Perilipin 1 (N-terminus) mouse monoclonal, PERI 112.17, supernatant

Cat. No: 651156

Available, delivery time 1-7 days

$238.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine, human and mouse
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Hybridoma cell culture supernatant
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone PERI 112.17
Immunogen Synthetic peptide of perilipin / PLIN1 (duplicated N-terminus of perilipin; aa 1-20)
Formulation Contains 0.09% sodium azide
UniprotID O60240 (Human), Q8CGN5 (Mouse)
Synonym Perilipin-1, Lipid droplet-associated protein, PLIN1, PERI, PLIN
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Background

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 kDa and 46 kDa, respectively. The N-terminus, however, remains unchanged.

The antibody reacts specifically with all PLIN1 variants located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, sebaceous gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It is also a useful pathological marker for steatogenesis e.g. in liver. It does not cross-react with adipophilin (ADRP, also named PLIN2) or TIP47 (also named PLIN3) proteins (or additional members of the PLIN/PAT-family, e.g. MLDP or OXPAT/PAT-1, also named PLIN5 or LSDP5).

Tested reactivity on cultured cell lines: several human carcinoma cell lines; 3T3-L1 adipocytes.


References/Publications (6)
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Category Protocol
Size 924.86 KB
Filetype pdf
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The anti-Perilipin 2,3,5 sample set provides antibodies directed against Perilipin 2, 3 and 5 to evaluate the presence and status in IHC and WB.

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  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse and rat

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anti-Perilipin 1 (N-terminus) guinea pig polyclonal, serum
Cat. No: GP29
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for IHC and WB
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