anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, liquid, purified, sample

1 ml (50 µg/ml)

200 µl (50 µg/ml)

50 µg

Cat. No: 690102S

Prices excl. tax

Available, delivery time: 1-7 days

$117.00*

Datasheet

Key Features

Purified, liquid, small
Mouse monoclonal
Suitable for IHC and WB
Reacts with dog, human and rat
Isotype: IgG1

Product description

Quantity	200 µl
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG1
Conjugate	Unconjugated
Application	IHC, WB
Purification	Affinity chromatography
Reactivity	Dog, Human, Rat
No reactivity	Bovine
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Clone	AP125
Immunogen	Synthetic peptide (aa 5-27 from N-terminus of human adipophilin/PLIN2)
Concentration	50 µg/ml (10 µg)
Formulation	PBS buffer, pH 7.4 with 0.09% sodium azide and 0.5 % BSA
UniprotID	A0A5F4CRI5 (Dog, Canis familiaris), Q99541 (Human), Q5U2U5 (Rat)
Synonym	Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:10-1:100 (0.5-5 µg/ml)
Immunohistochemistry (IHC) - paraffin	1:10-1:100 (0.5-5 µg/ml; microwave treatment recommended)
Western Blot (WB)	1:50-1:100 (0.5-1 µg/ml)

Background

Perilipin 2/Adipophilin/ADRP/PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir.
Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets.
Polypeptide reacting: Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.
Immunolocalization: Perilipin 2/Adipophilin/ADRP/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.Also positively stained are lipid-storing CD 68-positive macrophages.
Tested cultured cell lines: PLC, MDCK.

Learn more about PROGEN Perilipin antibodies.

References/Publications (43)

Publication

Species

Application

Publication Hayakawa, M. et al. Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma. Biochem. Biophys. Res. Commun. 640, 192–201 (2023).

Species human

Application ICC-IF

Publication Hara, Y. et al. A novel homozygous missense mutation in PNPLA2 in a patient manifesting primary triglyceride deposit cardiomyovasculopathy., Mol Genet Metab Rep 34, 100960, (2023).

Species human

Application IHC

Publication Kamerkar, S. et al. Metabolic and immune-sensitive contacts between lipid droplets and endoplasmic reticulum reconstituted in vitro., Proc Natl Acad Sci U S A 119, e2200513119, (2022).

Species rat

Application WB

Publication Schmitt D. et al. Lipid and protein content profiling of isolated native autophagic vesicles., EMBO Rep, 23, e53065, (2022).

Species human

Application WB

Publication Maeno, A. et al. A case of spontaneous Zymbal's gland carcinoma with lung metastasis in an aged Fischer 344 rat. J Toxicol Pathol. 34, 353-358(2021).

Species rat

Application IHC (paraffin)

Publication Moroki, T. et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol. 34, 161-180(2021).

Species rat

Application IHC

Publication Lassen, S. et al. Perilipin-2 is critical for efficient lipoprotein and hepatitis C virus particle production. J.Cell.Sci. 132, (2019)

Species human

Application WB

Publication Lenz, D. et al. SCYL1 variants cause a syndrome with low γ-glutamyl-transferase cholestasis, acute liver failure, and neurodegeneration (CALFAN). Genet.Med. , (2018).

Species human

Application IHC (paraffin)

Publication Jaisinghani, N. et al. Necrosis Driven Triglyceride Synthesis Primes Macrophages for Inflammation During Mycobacterium tuberculosis Infection. Front.Immunol. 9, 1490 (2018).

Species human

Application ICC-IF

Publication Kim, S. et al. PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC). Ocul.Surf. , (2018)

Species human

Application WB

Publication Bricambert, J. et al. The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity. Nat.Commun. 9, 2092 (2018)

Species mouse

Application WB

Publication Sadh, K. et al. Feeding-fasting dependent recruitment of membrane microdomain proteins to lipid droplets purified from the liver. PLoS.One. 12, e0183022 (2017).

Species rat

Application WB, IHC (frozen)

Publication Hirschmugl, B. et al. Maternal obesity modulates intracellular lipid turnover in the human term placenta. Int. J. Obes. 41, 317–323 (2017).

Species human

Application WB

Publication Ma, J. et al. An Immunohistochemical Investigation of Renal Phospholipidosis and Toxicity in Rats. Int.J.Toxicol. 36, 386-394 (2017).

Species rat

Application IHC(paraffin)

Publication Bianchi, C. et al. The glucose and lipid metabolism reprogramming is grade-dependent in clear cell renal cell carcinoma primary cultures and is targetable to modulate cell viability and proliferation. Oncotarget. 8, 113502-113515 (2017).

Species human

Application WB

Publication Ramos, S. V et al. Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb. Physiol. Reports 2, (2014).

Species rat

Application WB

Publication Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

Species human

Application ICC-IF

Publication Pfisterer, S. G. et al. Lipid droplet and early autophagosomal membrane targeting of Atg2A and Atg14L in human tumor cells. J. Lipid Res. 55, 1267-78 (2014).

Species human

Application WB,ICC-IF

Publication Macpherson, R. E. K., Vandenboom, R., Roy, B. D. & Peters, S. J. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physioligal Reports 1, (2013).

Species rat

Application WB

Publication Straub, B. K. et al. Adipophilin/perilipin-2 as a lipid droplet-specific marker for metabolically active cells and diseases associated with metabolic dysregulation. Histopathology 62, 617-631 (2013).

Species human

Application IHC (paraffin)

Publication MacPherson, R. E. K., Ramos, S. V, Vandenboom, R., Roy, B. D. & Peters, S. J. Skeletal muscle PLIN proteins, ATGL and CGI-58, interactions at rest and following stimulated contraction. Am. J. Physiol. Regul. Integr. Comp. Physiol. 304, R644-50 (2013).

Species rat

Application WB,Co-IP

Publication Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

Species human

Application WB,ICC-IF,IP

Publication Lin, P. et al. Efficient Lentiviral Transduction of Human Mesenchymal Stem Cells That Preserves Proliferation and Differentiation Capabilities. Stem Cells Transl. Med. 1, 886-897 (2012).

Species human

Application ICC-IF

Publication Dichlberger, A. et al. Lipid body formation during maturation of human mast cells. J. Lipid Res. 52, 2198-208 (2011).

Species human

Application WB,ICC-IF

Publication Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480-492 (2010).

Species human

Application WB,IHC

Publication Ostler, D. A. et al. Adipophilin expression in sebaceous tumors and other cutaneous lesions with clear cell histology: an immunohistochemical study of 117 cases. Mod. Pathol. 23, 567-573 (2010)

Species human

Application IHC (paraffin)

Publication Buers, I. et al. TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism. Arterioscler. Thromb. Vasc. Biol. 29, 767-773 (2009).

Species human

Application WB,ICC-IF

Publication Ingelmo-Torres, M. et al. Hydrophobic and Basic Domains Target Proteins to Lipid Droplets. Traffic 10, 1785-1801 (2009).

Species monkey

Application ICC-IF

Publication Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936-1946 (2008).

Species human

Application WB,IHC

Publication Muthusamy, K., Halbert, G. & Roberts, F. Immunohistochemical staining for adipophilin, perilipin and TIP47. J. Clin. Pathol. 59, 1166-1170 (2006).

Species human

Application IHC (paraffin)

Publication Robenek, H. et al. Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis. J. Cell Sci. 119, 4215-4224 (2006).

Species human

Application WB

Publication Robenek, H. et al. Butyrophilin controls milk fat globule secretion. Proc. Natl. Acad. Sci. USA 103, 10385-10390 (2006).

Species bovine

Application ICC-IF,IEM

Publication Larigauderie, G. et al. Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of ?-oxidation. FEBS J. 273, 3498-3510 (2006).

Species human

Application WB

Publication Robenek, H. et al. Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains*. J. Biol. Chem. 280, 26330-26338 (2005).

Species human

Application ICC-IF,IEM

Publication Robenek, H., Robenek, M. J. & Troyer, D. PAT family proteins pervade lipid droplet cores. J. Lipid Res. 46, 1331-1338 (2005).

Species human

Application IEM

Publication Ohsaki, Y., Maeda, T. & Fujimoto, T. Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins. Histochem. Cell Biol. 124, 445-452 (2005).

Species human

Application ICC-IF

Publication Robenek, H., Lorkowski, S., Schnoor, M. & Troyer, D. Spatial Integration of TIP47 and Adipophilin in Macrophage Lipid Bodies*. J. Biol. Chem. 280, 5789-5794 (2004).

Species human

Application WB,ICC-IF,IEM

Publication Fukumoto, S. & Fujimoto, T. Deformation of lipid droplets in fixed samples. Histochem. Cell Biol. 118, 423-428 (2002).

Species human

Application ICC-IF

Publication Ostermeyer, A. G. et al. Accumulation of Caveolin in the Endoplasmic Reticulum Redirects the Protein to Lipid Storage Droplets. J. Cell Biol. 152, 1071-1078 (2001).

Species human

Application ICC-IF

Publication Buechler, C. et al. Adipophilin is a sensitive marker for lipid loading in human blood monocytes. Biochim. Biophys. Acta 1532, 97-104 (2001).

Species human

Application WB

Publication Fujimoto, T., Kogo, H., Ishiguro, K., Tauchi, K. & Nomura, R. Caveolin-2 Is Targeted to Lipid Droplets, a New "Membrane Domain" in the Cell. J. Cell Biol. 152, 1079-1085 (2001).

Species human

Application WB

Publication Graham Hope, R. & McLauchlan, J. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J. Gen. Virol. 81, 1913-1925 (2000).

Species syrian hamster

Application WB

Publication Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).

Species human,rat,dog

Application IHC (frozen),ICC-IF,IEM

Downloads

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Category

Size

Filetype

File IHC mouse antibody

Category Protocol

Size 167.19 KB

Filetype pdf

File WB protocol

Category Protocol

Size 210.69 KB

Filetype pdf

Datasheet

MSDS

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FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.

The fixation method used for lipidic material influences tremendously the quality of staining results.
Tissue fixation should not exceed 2% paraformaldehyde.
Heat induced antigen retrieval is recommended. In many cases a standard protocol using 10 mM citrate buffer (pH 6) works fine.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The use of milk preparations (skim milk, dry milk) for blocking and for antibody dilution buffer might be problematic. The antigen of the Perilipin 2 antibodies was originally found and described in bovine milk and some dry milk brands (or even different lots) might contain the epitopes and block it, thus abolishing the reaction with the antigen transferred onto the Western blot membrane. In this case Tween 20 or BSA or ovalbumin is recommended as e.g. blocking material.
Immune reaction with cultured cells: this depends on the age of the cell culture; old cells (more than confluent) tend to accumulate fat and are therefore positive for Perilipin 2. Freshly split cells contain only few lipid droplets and are not easily detectable as positive for Perilipin 2.
Tissue with a high contents of fat might be problematic in electrophoretical separation. One has to load much material onto the SDS gel in order to get enough protein for a positive immunological signal. In addition the protein solubility is sometimes hindered by high fat concentration in the sample.

Positive signals from e.g. cultured cells depend on the age of the cell culture: old cells (more than confluent) tend to accumulate fat and are therefore positive for Perilipin 2. Freshly split cells contain only few lipid droplets and are not easily detectable as positive for Perilipin 2. With old /aged cells, however, there is always a problem with potential degradation processes.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Progen Support

For scientific and customer support, you can reach us by phone or email.