Disease related

anti-Perilipin 3 mouse monoclonal, 49.19, supernatant

Cat. No: 651168

Available, delivery time 1-7 days

$278.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 49.19
Immunogen Recombinant polypeptide of human TIP47 / PP17 / PLIN3
Formulation Contains 0.09% sodium azide
UniprotID Q3SX32 (Bovine),O60664 (Human),Q9DBG5 (Mouse),M0RA08 (Rat)
Synonym Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, PP17, PLIN3, M6PRBP1, TIP47
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Undiluted - 1:10 (also after fixation with 2% PFA)
Immunohistochemistry (IHC) - paraffin Undiluted - 1:10 (microwave treatmtent recommended)
Western Blot (WB) Assay dependent (avoid use of milk proteins (e.g. dry milk preparations) in blocking and incubation buffers)
Background

TIP47 (tail-interacting protein of 47 kDa; also named Perilipin 3, PLIN3) is involved in lipid droplet maturation (see e.g. Bulankina 2003 and 2009). The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47/PLIN3 are secreted into circulation of cervix carcinoma patients. After radical surgery PLIN3 serum levels are decreased (cf. Than et al. 1999).
TIP47/PLIN3 shows about 40% sequence homology to adipophilin (PLIN2). The antibody, however, is specific for TIP47/PLIN3 and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other proteins of the PLIN/PAT family.
Reactivity on cultured cell lines: PLC, A431, HT29, HeLa, SV-80; human pre-adipocytes

Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641-55 (2009).
Bulankina, A. V. TIP47 is recruited to lipid droplets and important for the organelle biogenesis and function. Computing (2003).
Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): Homology with adipophilin and the mouse adipose, differentiation-related protein. Tumor Biol. 20, 184-192 (1999).

References/Publications (1)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
MFGM (human or bovine) from milk
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
The use of milk preparations (skim milk, dry milk) for blocking (and also for antibody dilution buffer) might be problematic. The antigens of Perilipin 3 antibodies were originally found and described in bovine milk. Some dry milk brands (or even different lots) might contain the epitopes and block it, thus abolishing the reaction with the antigen transferred onto the Western blot membrane and/or causing background staining on the transfer membrane itself. In this case Tween 20 or BSA or ovalbumin is recommended as e.g. blocking material.
anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, liquid, purified, sample
Cat. No: 690102S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with dog, human and rat
  • Isotype: IgG1

$117.00*
anti-Perilipin 1 (N-terminus) mouse monoclonal, PERI 112.17, liquid, purified, sample
Cat. No: 690156S
Quantity: 200 µl (100 µg/ml)
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with human and mouse
  • Isotype: IgG1

$120.00*
anti-Perilipin 5 mouse monoclonal, MLDP-130.336, supernatant
Cat. No: 651176
  • Mouse monoclonal
  • Suitable for IEM, IHC and WB
  • Reacts with human
  • Isotype: IgG1

$278.00*
anti-Perilipin 1 (C-terminus) guinea pig polyclonal, serum
Cat. No: GP33
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse and rat

$299.00*
anti-Perilipin 3 (mouse C-Terminus) guinea pig polyclonal, serum
Cat. No: GP37
  •     Guinea pig polyclonal
  •     Suitable for IHC and WB
  •     Reacts with mouse and rat

$333.00*
anti-Perilipin 4 (N-terminus) guinea pig polyclonal, serum
Cat. No: GP38
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human

$333.00*
anti-Perilipin 3 (mouse N-Terminus) guinea pig polyclonal, serum
Cat. No: GP36
  •     Guinea pig polyclonal
  •     Suitable for IHC and WB
  •     Reacts with mouse and rat

$333.00*
anti-Perilipin 3 (C-terminus) guinea pig polyclonal, serum
Cat. No: GP32
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human

$333.00*
anti-Perilipin 5 (N-terminus) guinea pig polyclonal, serum
Cat. No: GP44
  • Guinea pig polyclonal
  • Suitable for ICC/IF and IHC
  • Reacts with human

$333.00*
anti-Perilipin 2 (N-terminus aa 1-16) guinea pig polyclonal, serum
Cat. No: GP46
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

$356.00*
anti-Perilipin 2 (C-terminus) guinea pig polyclonal, serum
Cat. No: GP41
  • Guinea pig polyclonal
  • Suitable for IHC, ICC/IF and WB
  • Reacts with human

$356.00*
anti-Perilipin 2 (mouse N-terminus) guinea pig polyclonal, serum
Cat. No: GP42
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with mouse

$356.00*
anti-Perilipin 2 (N-terminus aa 6-27) guinea pig polyclonal, serum
Cat. No: GP47
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

$356.00*