- Mouse monoclonal
- Suitable for ELISA, FACS, ICC/IF, IHC and WB
- Reacts with human
- Isotype: IgG1 kappa
Product description
| Quantity | 1 ml (100 µg/ml) |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 kappa |
| Conjugate | Unconjugated |
| Application | ELISA, FACS, ICC/IF, IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Human |
| No reactivity | Mouse |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | EBS-O-175 |
| Immunogen | Human rTNF-alpha |
| Concentration | 100 µg/ml |
| Formulation | PBS with 0.02% sodium azide |
| UniprotID | P01375 (Human) |
| Synonym | Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a [Cleaved into: Tumor necrosis factor, membrane form, N-terminal fragment, NTF; Intracellular domain 1, ICD1; Intracellular domain 2, ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form], TNF, TNFA, TNFSF2 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:50-1:100 (1-2 µg/ml) |
| Immunohistochemistry (IHC) - frozen | 1:50-1:100 (1-2 µg/ml) |
| ELISA | Assay dependent |
| Flow Cytometry (FACS) | 0.5-1.0 µg/million cells in 0.1 ml |
| Western Blot (WB) | 1:50-1:100 (1-2 µg/ml) |
Background
EBS-O-175 reacts with human TNF-alpha and not with mouse TNF-alpha. This antibody neutralises Hu rTNF-alpha mediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of Hu rTNF-alpha.
Positive control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil.
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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