anti-p16 Protein mouse monoclonal, DCS-50, lyophilized, purified
- Purified, lyophilized
- Mouse monoclonal
- Suitable for FACS, IP, ICC/IF, IHC and WB
- Reacts with human
- Isotype: IgG1
|Application||FACS, ICC/IF, IHC, IP, WB|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Recombinant human p16 protein|
|Concentration||50 µg/ml after reconstitution with 1 ml dist. water
|Formulation||Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)|
|Synonym||Putative protein CDKN2A-DT, CDKN2A antisense RNA 1, CDKN2A antisense gene protein 1, Protein CDKN2A-AS1, Susceptibility protein NSG-x, CDKN2A-DT, C9orf53, CDKN2A-AS1|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:10|
|Immunohistochemistry (IHC) - paraffin||1:10 (microwave treatment recommended)|
|Flow Cytometry (FACS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
p16 (CDKN2A, p16Ink4A), is key regulator of the cell cycle and involved in cell cycle control and cellular senescence. It is a specific inhibitor for Cdk4 and Cdk6 and binds to the phosphorylated Cdk-cyclin complex. A disruption of this pathway is commonly observed in cancer.
p16 is lost in the majority of tumor cell lines and in most primary tumors. It is not expressed in melanoma. In carcinoma driven by an HPV (human papilloma virus) infection, p16 is often overexpressed.
The antibody is especially useful for immunoprecipitation.
The epitope was localized within the 15 aa residues of the C-terminus of p16 protein.
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