Epitope mapped
Diagnostic marker

anti-p16 Protein mouse monoclonal, DCS-50, lyophilized, purified

Lyophilized

Cat. No: 61074

Available, delivery time 1-7 days

$232.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for FACS, IP, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, IP, WB
Purification Affinity chromatography
Reactivity Human
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone DCS-50
Immunogen Recombinant human p16 protein
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID Q9UH64 (Human)
Synonym Putative protein CDKN2A-DT, CDKN2A antisense RNA 1, CDKN2A antisense gene protein 1, Protein CDKN2A-AS1, Susceptibility protein NSG-x, CDKN2A-DT, C9orf53, CDKN2A-AS1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Flow Cytometry (FACS) Assay dependent
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) Assay dependent
Background

p16 (CDKN2A, p16Ink4A), is key regulator of the cell cycle and involved in cell cycle control and cellular senescence. It is a specific inhibitor for Cdk4 and Cdk6 and binds to the phosphorylated Cdk-cyclin complex. A disruption of this pathway is commonly observed in cancer.
p16 is lost in the majority of tumor cell lines and in most primary tumors. It is not expressed in melanoma. In carcinoma driven by an HPV (human papilloma virus) infection, p16 is often overexpressed.
The antibody is especially useful for immunoprecipitation.
The epitope was localized within the 15 aa residues of the C-terminus of p16 protein.

References/Publications (3)
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